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Use of 3DCellMaker
Microscope photos displaying various cells being cultured in 3DCellMaker.
click images for full size
F1: Cell Type: MCF-7
Seeding Density: 100,000 cells/ml
Thermogel: Prewarmed (gelled) A3DC
Growth 4 days after seeding
F2: Cell Type: MCF-7
Seeding Density: 100,000 cells/ml
Thermogel: Prewarmed (gelled) A3DH
Growth 24 hours after seeding
F3: Cell Type: CCD-1068SK fibroblasts
Seeding density: 50,000 cells/mL
Thermogel: Prewarmed (gelled) A3DC
Growth after 24 hours after seeding
F4: Cell Type: CCD-1068SK fibroblasts
Seeding density: 125,000 cells/ml
Thermogel: Prewarmed (gelled) A3DH
Growth 24 hours after seeding
F5: Cell Type: HEP G2
Seeding density: 150,000 cells/ml
Thermogel: Cold A3DC
Growth 14 days after seeding
F6: Cell Type: HEP G2
Seeding density: 100,000 cells/ml
Thermogel: Prewarmed (gelled) A3DH
Growth 24 hours after seeding
F7: Cell Type: VERO
Seeding density: 250,000 cells/ml
Thermogel: Cold A3DC
Growth 4 days after seeding
F8: Cell Type: VERO
Seeding density: 250,000 cells/ml
Thermogel: Cold A3DC
Growth 2 days after seeding
Use of 3DCellMaker in Microfluidic Device
Below are a series of microscope photos displaying MCF-7 cells being cultured on a TMOC (Tumor Microenvironment on Chip)
microfluidic platform developed by Bumsoo Han Ph.D and his team at Purdue University. We used this device to culture MCF-7
cells in 3DCellMaker. In this experiment we used A3DH for its ability to thermogel quickly once the microfluidic device
was filled. We do not recommend the use of A3DH in cold seeding applications but it was a viable option in this instance.
click images for full size
F9: MCF-7 in A3DH immediately after filling tumor channel (40X magnification).
F10: MCF-7 in A3DH 2 hours after filling tumor channel and incubated at 37°C during this time (40X magnification). Cells have already started to aggregate.
F11: MCF-7 in A3DH 24 hours post filling of tumor channel (40X magnification).
F12: MCF-7 in A3DH 48 hours post filling of tumor channel (40X magnification).
F13: MCF-7 in A3DH 6 days post filling of tumor channel (40X magnification).
F14: MCF-7 in A3DH 9 days post filling of tumor channel. Media was replaced before photo which caused some of the spheroids to be drawn to edge of tumor channel(40X magnification).
