Akina
Justin Hadar's Technical Blog
Justin HadarJustin Hadar,
Senior Scientist

3DCellMaker

A blog dedicated to addressing technical questions relating to 3DCellMaker.


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3D Cell Culture Exhibits Clear Advantages in Evaluating Drug Sensitivity and Resistance Compared to 2D Monolayer.

Tuesday, August 23, 2016, 9:06 AM ET


3DCellMaker (www.3dcellmaker.com) is a product from PolySciTech division of Akina, Inc. that allows for 3D growth of cells.

Recently researchers using HER2-positive breast cancer cell lines (BT474, HCC1954, EFM192A) displayed increased resistance to both HER targeted and classical chemotherapeutics. They also showed that protein expression involved in cell survival and transporters associated with drug resistance were increased in 3D cultures as compared to 2D monolayer cultures. This research is important to the development of better therapies for drug resistant cancer lines. Read more here: Breslin, Susan, and Lorraine O'Driscoll. "The relevance of using 3D cell cultures, in addition to 2D monolayer cultures, when evaluating breast cancer drug sensitivity and resistance." Oncotarget 7, no. 29 (2016): 45745-45756. www.tara.tcd.ie/bitstream/handle/2262/76500/Breslin%20%26%20ODriscoll_Oncotarget-2016.pdf?sequence=1&isAllowed=y

Summary:
"Solid tumours naturally grow in 3D wherein the spatial arrangement of cells affects how they interact with each other. This suggests that 3D cell culture may mimic the natural in vivo setting better than traditional monolayer (2D) cell culture, where cells are grown attached to plastic. Here, using HER2-positive breast cancer cell lines as models (BT474, HCC1954, EFM192A), the effects of culturing cells in 3D using the poly-HEMA method compared to 2D cultures were assessed in terms of cellular viability, response/resistance to anti-cancer drugs, protein expression and enzyme activity. Scanning electron microscopy showed the morphology of cells in 3D to be substantially different to those cultured in 2D. Cell viability in 3D cells was substantially lower than that of cells in 2D cultures, while 3D cultures were more resistant to the effects of HER-targeted (neratinib) and classical chemotherapy (docetaxel) drugs. Expression of proteins involved in cell survival, transporters associated with drug resistance and drug targets were increased in 3D cultures. Finally, activity of drug metabolising enzyme CYP3A4 was substantially increased in 3D compared to 2D cultures. Together this data indicates that the biological information represented by 3D and 2D cell cultures is substantially different i.e. 3D cell cultures demonstrate higher innate resistance to anti-cancer drugs compared to 2D cultures, which may be facilitated by the altered receptor proteins, drug transporters and metabolising enzyme activity. This highlights the importance of considering 3D in addition to 2D culture methods in pre-clinical studies of both newer targeted and more traditional anti-cancer drugs."


How to use 3DCellMaker

Friday, August 5, 2016, 11:26 AM ET


A short video showing how to use 3DCellMaker



Research Samples Available

Friday, August 5, 2016, 8:22 AM ET


PolySciTech is pleased to announce that we will be offering free samples of 3DCellMaker.


In an effort to establish a database of properties for the material including cell-line response, culture techniques, and application notes, Akina, Inc. provides research samples of material to select applicants for obtaining useful data, as part of the commercialization of the material.

For researchers who have an interest in testing our product in their chosen application. Below is a link to a research sample application form.

Research Sample Application Form


2016 CRS

Tuesday, August 2, 2016, 7:14 AM ET



PolySciTech recently attended the 2016 Controlled Release Society convention in Seattle, WA July 17-July 20 to present our research on 3DCellMaker. We presented this research as both a poster presentation and as a research highlight talk. We would like to thank everyone that stopped by the poster or our booth to discuss our exciting new product. Below is a link to our poster.

2016CRS-3DCellMaker




Photos of Cell Culture in 3DCellMaker

Tuesday, August 2, 2016, 6:05 AM ET


Cell Type: MCF-7
Seeding Density: 100,000 cells/ml
Thermogel: Prewarmed (gelled) A3DC
Growth 4 days after seeding

Cell Type: MCF-7
Seeding Density: 100,000 cells/ml
Thermogel: Prewarmed (gelled) A3DH
Growth 24 hours after seeding

Cell Type: CCD-1068SK fibroblasts
Seeding density: 125,000 cells/ml
Thermogel: Prewarmed (gelled) A3DH
Growth 24 hours after seeding

Cell Type: CCD-1068SK fibroblasts
Seeding density: 50,000 cells/mL
Thermogel: Prewarmed (gelled) A3DC
Growth after 24 hours after seeding

Cell Type: VERO
Seeding density: 250,000 cells/ml
Thermogel: Cold A3DC
Growth 2 days after seeding

Cell Type: VERO
Seeding density: 250,000 cells/ml
Thermogel: Cold A3DC
Growth 4 days after seeding

Cell Type: HEP G2
Seeding density: 100,000 cells/ml
Thermogel: Prewarmed (gelled) A3DH
Growth 24 hours after seeding

Cell Type: HEP G2
Seeding density: 150,000 cells/ml
Thermogel: Cold A3DC
Growth 14 days after seeding


Introducing 3DCellMaker

Tuesday, August 2, 2016, 6:05 AM ET


3DCellMaker is a fully synthetic, reproducible thermogelling polymer which enables cancer cells as well as normal state cells to grow into physiologically relevant 3D spheroids. This growth occurs regardless of cell culture techniques and can be done in a typical, flat well plate or dish using conventional culture media with FBS supplement (or serum free media) in any 5% CO2, 37°C incubator.

PolySciTech a division of Akina offers two versions of 3DCellMaker

  • 3DCellMaker A3DC Solution Seeded:
    Purified, sterile-packed (poloxamer 407)-polyurethane for mixing cells in cold media solution and warming to form a gel.
  • 3DCellMaker A3DH Gel Seeded:
    Purified, sterile-packed, stearate-modified methyl cellulose for placing cells on top of already formed gel.
See the website for sales.


These posts are syndicated from Justin Hadar's blog at 3dcellmaker.blogspot.com/.

 

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